This method includes the hormonal stimulation of the ovaries according to the established protocol, followed by the egg collection. The eggs are fertilised outside of the patient’s body - in vitro. The fertilized eggs are cultivated in a special laboratory environment, where we ensure the embryos develop optimally. The chosen embryo is then implanted into the patient’s uterus. The length of the entire process, starting with the initial consultation up until the embryo transfer, differs with regards to the patient’s menstrual cycle. An indication of the estimated time may be somewhere between 1 to 2.5 months.

With this method, we use freshly collected eggs from an anonymous donor, which are fertilized by the partner’s sperm. The preparation and synchronization of the patient and donor can take approximately 2 months. The patient only uses medication for the endometrium preparation for the embryo transfer, they will not undergo hormonal stimulation. The protocol for the donors at IVF CUBE is as follows; we only accept donors between the ages of 20-34 after they undergo the examinations and testing required by law. Selection of the donor is based on phenotypic criteria according to the couple’s features and their requirements. In addition to the state required testing, our donors are also examined by our clinical psychologist. Information from the psychological profile developed by the psychologist is also available for the patient upon request. The guarantees of the program are: a minimum of 8 eggs and at least 2 high quality embryos in the blastocyst stage (if the partner's sperm is normozoospermia). Find out more about the treatment process.

A micromanipulation method in which the protein cover (pellucid zone) of the egg is opened by laser. The hatching process is then facilitated by the opening of the cover in the inner cellular part so that the embryo can easily get through the pellucid zone. Assisted hatching is performed on all the available embryos on day 4, when embryos reach the compact stage.

A method of quick freezing (of embryos, eggs, sperm). Quick freezing is much more compatible to the human cells, because it prevents any crystals forming and increases the safety of the cells with further unfreezing and usage in the following IVF cycles. Vitrification is a standard part of all cycles performed at IVF CUBE.

In cases where it is impossible to use the partner’s sperm, it is possible to use donor sperm from an anonymous donor. The clinic chooses the donors from our own large donor bank that includes more than 200 donors.The donors are always chosen according to set criteria required by the couple.

Is a technique offered to clients as a supportive treatment for IVF treatment. Acupuncture stimulates the energetic areas that are specially chosen for the assisted reproduction cycles. The procedure is performed 30 minutes before the embryo transfer and 30 minutes after. The main goal is to promote the blood circulation of the endometrium and to ease the pelvic floor. In addition to the benefits mentioned above, it also has a positive effect on the patient’s psyche.

For all cases we offer the possibility of an initial free psychological consultation with our experienced psychologist. M.Ed. Jana Brezinova specialises in couples therapy, but also assists in individual therapy for our IVF patients. The psychological consultations help to improve and stabilise the psychological well-being of our clients, which can be burdened during the demanding and often long infertility therapies. The psychological aspect can play an important role and can be one of the crucial factors influencing the success rate.

Infertility problems are, according to the statistics, equally caused by male and female problems. That is why at IVF CUBE we always investigate both male and female partners.

Female infertility investigation: The basic examination involves testing the blood for AMH (Anti-Mullerian Hormone) and ultrasound scanning for the AFC (Antral Follicle Count). On the basis of these two examinations we are able to predict ovarian reserves and establish the prognosis for the fertilisation. In cases where the results show abnormalities, it is possible to undergo more testing or treatment for the fertility malfunction.

Male infertility investigation: After we have received the sperm sample, we perform a sperm analysis examination. In this testing we evaluate and compare the levels with the WHO (World Health Organisation) standards. The parameters we evaluate are the volume, motility, sperm morphology and many others. Further information can be found in the Infertility Investigation section.

A method designed for women that wish for whatever reason to preserve a good amount of eggs for further use. One of the significant indications for egg freezing may be oncological treatment, which is known to be highly toxic for the reproductive cells. Other reasons may be the purposeful postponement of maternity or the fact that a woman has not found the right life partner yet and is aware of decreasing fertility with aging. In the case of egg vitrification the patient undergoes the first part of the treatment, through the stimulation and egg retrieval. After the collection the eggs are cryopreserved in tanks with liquid nitrogen here at IVF CUBE.

The procedure allows long term, almost unlimited conservation of human reproductive cells. The cells are stored at -196 C (-321 F). Cryopreservation does not cause any harm to sperm so when it comes to fertilisation the difference between frozen and fresh sperm is extremely minimal.The cryopreservation of the sperm is applicable especially to the male partners that have unstable sperm analysis results, limited sperm count in ejaculate or for males who suffer from a psychological blockage when providing the samples. Another indication might be planned oncological treatment.

 

4D ultrasound examination enables a moving three-dimensional visual display of the baby in the womb in real time. In earlier stages of pregnancy, this technique projects the entire baby; in later stages, the images display primarily spatial representations of individual body parts, particularly the face. At our clinic, IVF CUBE, you can get a free examination recorded on a flash drive.

Extensive sperm diagnosis SPERM+ provides an early detection of possible infertility causes in male partner. The screening consists of 4 laboratory tests. Each of these examines crucial factors causing conception problems or issues with embryo development. SPERM+ diagnosis enables us to determine the most suitable method of sperm selection and its processing.

• Sperm DNA Fragmentation (HALO sperm test)
• Sperm DNA fragmentation is a common cause of infertility in male partner. The test checks for damages in a spermatozoon head as each of the heads carry vital genetic information. Effective embryo development relies on healthy spermatozoon.
• Vitality Test
• This test is able to determine viable spermatozoons and it is particularly useful in suspected low quality sperm samples.
• Oxidative Stress Level
• The ejaculate contains traces of oxygen and nitrogen, which have to be balanced in order to preserve physiological functions. Increased concentration of either is called oxidative stress which impacts negatively on sperm DNA.
• Presence of Antisperm Antibodies
• Infertility can also be caused by immunological factors. Testing is performed to determine whether specific antibodies are present.